PCR-melting curve method

  The main principle of PCR fluorescent probe melting curve technology is to design molecular beacon probes for the sites to be detected; after PCR amplification is completed, the amplified products are hybridized with molecular beacon probes, then slowly rise Temperature, do the melting curve analysis of the probe and the product, and read the genetic polymorphism information of the site according to the temperature corresponding to the melting peak. Compared with other methods, it has the advantages of simple operation, high accuracy, good specificity and easy to judge results.